Mitigating effect of clopidogrel and systematic management on adverse events after interventional therapy for coronary heart disease

Purpose: To explore the molecular targets of Guanxin Danshen Dripping Pills (GXDS) in regulating psycho-cardiology disease through identifying its components by UPLC-MS/MS and network pharmacology approach.
 Methods: Ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was adopted to determine GXDS’ main constituents. SwissTargetPrediction similarity ensemble approach were employed predict possible targets. Targets for psycho-cardiological identified from GeneCards database. The GXDS overlapping these using Venny web tool (version 2.1.0). Functional enrichment analysis performed Gene Ontology knowledgebase, while Kyoto Encyclopedia Genes Genomes (KEGG) pathways undertaken. In Cytoscape, target-pathway, pathway-target, compound-target networks constructed.
 Results: Among 37 chemical UPLC-MS/MS, 19 combined with 63 targets, which subjected gene ontology obtain terms corresponding sub-ontologies cellular components, functions, biological processes (30, 84, 1188, respectively). revealed 109 related pathways.
 Conclusion: This study them analysis, explored material basis therapeutic effect on disease. Thus, is a potential candidate use clinical treatment diseases